Description

categories- Leukemia cell lines-cryovial-organism-Human-tissue-Peripheral blood-disease–synonyms-RPMI-1788, RPMI1788, Roswell Park Memorial Institute 1788, GM02131, GM2131, GM02131A, GM17219-age-33 years-gender-Male-ethnicity-Caucasian-morphology-Round cells-cellType-B lymphoblast-growth Properties-Suspension-RPMI 1788 (CLS catalog number 300318)-biosafety Level-2-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Start new cultures at 3 x 10^5 viable cells/ml and subculture at 1 x 10^6 cells/ml. Prepare dilutions by transferring the appropriate amount of cell suspension into new flasks with fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)