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M-MSV-Balb/3T3

Mouse cell lines

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Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Embryonic-disease–synonyms-M-MSV-BALB/3T3-age-Embryo, 14 to 17 day gestation-gender-Female-ethnicity–morphology-Fibroblast-like-cellType-Fibroblast-growth Properties-Adherent-M-MSV-Balb/3T3 (CLS catalog number 400458)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)

Brand

CYTION