Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue–disease–synonyms-HB-222-age–gender–ethnicity–morphology-Lymphoblast-cellType–growth Properties-Suspension-35.1 (CLS catalog number 305002)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks.-freezeMedium-CM-1 (CLS catalog number 800150)