Description

categories-Lung cancer cell lines-cryovial-organism-Human-tissue-Lung-disease-Pleural Mesothelioma-synonyms-H-Meso-1, HMESO-1, HMeso-1, HMeso1, HMESO1, H-Meso, HMESO, Hmeso, Hmeso-age-35 years-gender-Male-ethnicity-Caucasian-morphology-Epithelial-like-cellType–growth Properties-Adherent-H-MESO-1 (CLS catalog number 300186)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)