Description
categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Peripheral blood-disease-Leukemia-synonyms-WEHI-3b, Wehi-3B, WEHI 3B, WEHI3B-age–gender–ethnicity–morphology–cellType-Myelomonocyte-growth Properties-Suspension-WEHI-3B (CLS catalog number 400376)-biosafety Level-2-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10^5 cells/ml and maintain between 1 x 10^5 and 1×10^6 cells/ml. Adherent cells can be recovered by scraping.-freezeMedium-CM-1 (CLS catalog number 800150)
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