Description
categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Blood-disease-Leukemia-synonyms–age-Adult-gender-Unspecified-ethnicity–morphology-Lymphocytic-cellType–growth Properties-Suspension-EGG (CLS catalog number 400171)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 1 mM sodium pyruvate-subculturing-Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1×10^5 cells/ml with fresh medium and transfer into new flasks.-freezeMedium-CM-ACF (CLS catalog number 800650)
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