Description
categories- Breast cancer cell lines-cryovial-organism-Human-tissue-Mammary gland, breast-disease-Invasive breast carcinoma-synonyms-HS 578T, Hs-578T, HS-578T, Hs_578t, Hs-578-T, HS-578-T, Hs 578.T, HS578T, Hs578T, Hs578t, HS0578T, 578T, HS578, Hs578, Homo sapiens No. 578, tumor cells-age-74 years-gender-Female-ethnicity-European-morphology-Epithelial-cellType–growth Properties-Adherent-Hs 578T (CLS catalog number 305089)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 0.01 mg/ml human insulin-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)
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