Description

categories-B lymphoblastoid cell lines-cryovial-organism-Human-tissue-Peripheral blood-disease–synonyms-WT-51, WT 51, GM03103, GM3103, GM03103A-age-Unspecified-gender-Male-ethnicity-Caucasian-morphology-Round cells-cellType-B lymphoblast-growth Properties-Suspension-WT51 (CLS catalog number 302141)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Take an aliquot of the cell suspension to determine the cell concentration, then carefully resuspend the cells and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)