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Canine cell lines

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categories-Canine cell lines-cryovial-organism-Canine-tissue-Kidney-disease–synonyms-MDCK, NBL-2, Madin-Darby Canine Kidney, Madin Darby Canine Kidney-age-Adult-gender-Female-ethnicity–morphology-Epithelial-like-cellType-Epithelial-growth Properties-Monolayer, adherent-MDCK (NBL-2) (CLS catalog number 602280)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-5% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)