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Bovine cell lines

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categories-Bovine cell lines-cryovial-organism-Bovine-tissue-Kidney-disease–synonyms-MDBK (NBL-1), NBL-1, Madin-Darby Bovine Kidney, Madin Darby Bovine Kidney-age-Adult-gender-Male-ethnicity–morphology-Epithelial-like-cellType–growth Properties-Monolayer, adherent-MDBK (NBL-1) (CLS catalog number 600396)-biosafety Level-1-cultureMedium-EMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 0.1 mM NEAA, 1 mM sodium pyruvate-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)