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Human cell lines

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categories- Human cell lines-cryovial-organism-Human-tissue-Mammary gland, breast-disease–synonyms-MCF 10A, MCF.10A, MCF10A, MCF10-A, MCF10a, MCF-10 Attached, Michigan Cancer Foundation-10A-age-36 years-gender-Female-ethnicity–morphology-Epithelial-cellType–growth Properties-Adherent-MCF 10A (CLS catalog number 305026)-biosafety Level-1-cultureMedium-MEGM-mediumSupplements-100 ng/ml cholera toxin-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)