Description

categories- Lymphoma-cryovial-organism-Human-tissue-Blood-disease-T-cell acute lymphoblastic leukemia-synonyms-JURKAT, JM, JM-Jurkat, Jurkat-FHCRC, Jurkat FHCRC, FHCRC-11, FHCRC subclone 11, FCCH1024-age-14 years-gender-Male-ethnicity-European-morphology-Lymphoblast-cellType–growth Properties-Suspension-Jurkat (CLS catalog number 302147)-biosafety Level-1-cultureMedium-IMEM-mediumSupplements-10% FBS, 0.05 mM 2-mercaptoethanol-subculturing-Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks.-freezeMedium-CM-1 (CLS catalog number 800150)