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Head-Neck cancer cell lines

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categories-Head-Neck cancer cell lines-cryovial-organism-Human-tissue-Esophagus-disease-Squamous cell carcinoma-synonyms-COLO 680N, COLO #680N, COLO680N, Colorado 680N-age-57 years-gender-Female-ethnicity-African-morphology-Epithelial-like-cellType–growth Properties-Monolayer, adherent-Colo-680N (CLS catalog number 300464)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)