Description
categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Skin-disease–synonyms-B82 (Hamprecht), B-82, GM00347, GM-347, GM0347, GM347, GM00347A, GM 0347A-age-100 days-gender-Male-ethnicity–morphology-Fibroblast-cellType–growth Properties-Adherent-B82 (CLS catalog number 305173)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 1 mM 8-bromodeoxyuridine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)
