Description

Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays[1][2][3].-4°C (Powder, protect from light)-Applications–Formula-C17H19N3-Citation–References-[1]Darzynkiewicz Z, et al. Differential staining of DNA and RNA. Curr Protoc Cytom. 2004 Nov;Chapter 7:Unit 7.3.|[2]Mirrett S. Acridine orange stain. Infect Control. 1982 May-Jun;3(3):250-2. |[3]Yektaeian N, et al. Lipophilic tracer Dil and fluorescence labeling of acridine orange used for Leishmania major tracing in the fibroblast cells. Heliyon. 2019 Dec 18;5(12):e03073. -CASNumber-494-38-2-MolecularWeight-265.35-Compound Purity–SMILES-CN(C)C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1-Research_Area-Others-Solubility-10 mM in DMSO-Target-Parasite-Isoform–Pathway-Anti-infection-MCE Product type-Dye Reagents