Description

For quantitative determination of NADP+/NADPH concentrations and ratios in cell or tissue extracts. Key Features: Sensitive and accurate. Detection limit 0.1 µM, linearity up to 10 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Colorimetric Determination at 565 nm. Samples: Cells, Tissue. Species: human, mouse, rat and more. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 µM.