Description

For assessing PDH activity in biological samples and screening and evaluation of PDH modulators. High sensitivity and wide linear range. The detection limit is 0.87 U/L, with linearity up to at least 278 U/L PDH in a 96-well plate assay. Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of PDH activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD565nm; Samples: Biological samples; Procedures: 20 min; Detection Limit: 0.87 U/L