Description

categories-Mesenchymal Stem cells-cells-organism-Human-tissue-Umbilical Cord – Artery-disease–synonyms–age-Please inquire-gender-Please inquire-ethnicity-Caucasian-morphology-Well-spread spindle shaped, fibroblast-like morphology for at least within 5 passages. Fewer than 2% cells exhibit spontaneous myofibroblast-like morphology within each passage.-cellType-Stem cell-growth Properties-Adherent-Human Mesenchymal Stem Cells, Whartons Jelly (HMSC-WJ) (CLS catalog number 300685)-biosafety Level-1-cultureMedium-Mesenchymal Cell Growth Medium X1-mediumSupplements–subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)