Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Kidney-disease–synonyms–age-Adult-gender-Unspecified-ethnicity–morphology–cellType-Podocyte-growth Properties-Adherent-E11 (CLS catalog number 400425)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature (do not exceed 33?C) for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.-freezeMedium-CM-ACF (CLS catalog number 800650)