Description

categories-Fibroblasts-cryovial-organism-Human-tissue-Dermis-disease–synonyms–age–gender–ethnicity-Caucasian-morphology–cellType–growth Properties-Adherent-Normal Human Dermal Fibroblast (CLS catalog number 300606)-biosafety Level-1-cultureMedium-MEM without ribonucleosides and deoxyribonucleosides, 10% FBS, 2 ng/ml hr-bFGF, 2 mM stable L-glutamine.-mediumSupplements–subculturing-1. Remove the culture medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). 2. Add trypsin-EDTA 0.25% solution, 1-2ml per T25, 2.5ml per T75 cell culture flask, the cell sheet must be covered completely, and incubate at 37?C for 10 min. 3. Stop the trypsin activity using FBS-containing cell culture medium. Dispense into new flasks which contain fresh cell culture medium.-freezeMedium-CM-1 (CLS catalog number 800150)