Description
categories-Mouse cell lines-cryovial-organism-Mouse-tissue–disease-Sarcoma-synonyms-Sarcoma 180-age–gender–ethnicity–morphology-Fibroblast-like-cellType–growth Properties-Adherent-Sarcoma-180 (CLS catalog number 400330)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Start cultures at 2 x 10^5 cells/ml and maintain between 1 x 10^5 and 1 x 10^6 cells/ml. Subculture by transferring an appropriate number of cells into fresh cell culture flasks filled with fresh cell culture medium.-freezeMedium-CM-1 (CLS catalog number 800150)
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