Description

categories-Rat cell lines-cryovial-organism-Rat-tissue-Liver-disease-Rat hepatocellular carcinoma-synonyms-H4II, H-35tc2, Reuber-H-35 hepatoma tissue culture 2, Reuber H-35 tc2, Reuber H35 tc2, H-35 Reuber tc2, H35 Reuber tc2, RH-35 tc2, RH35 tc2, H-35 tc2, H35 tc2-age–gender-Male-ethnicity–morphology-Epithelial-cellType–growth Properties-Adherent-RH-35 (CLS catalog number 305210)-biosafety Level-1-cultureMedium-Coons modified Hams F12-mediumSupplements-5% FBS, 2mM Glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)