Description

categories- Brain cancer cell lines-cryovial-organism-Human-tissue-Brain-disease-Oligodendroglioma-synonyms-HS 683, Hs 683, Hs-683, Hs683, HS683, Hs 683.T, HS 683T, Hs683T-age-76 years-gender-Male-ethnicity-Caucasian-morphology-Fibroblast-like-cellType–growth Properties-Adherent-HS-683 (CLS catalog number 300213)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature (about 20-26?C) for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)