Description
categories- Leukemia cell lines-cryovial-organism-Human-tissue-Blood-disease-Mycosis fungoides and Sezary syndrome-synonyms-Hut 78, HUT 78, HuT 78, HUT-78, HuT78, Hut78, HUT78, NCI-H78-age-53 years-gender-Male-ethnicity-Caucasian-morphology-Round cells-cellType-T lymphoblast-growth Properties-Suspension-HuT-78 (CLS catalog number 300338)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Start cultures at 1 x 10^5 cells/ml and maintain between 1 x 10^5 and 1 x 10^6 cells/ml. Subculture by pipetting aliquots into new cell culture flasks containing the appropriate amount of cell culture media.-freezeMedium-CM-1 (CLS catalog number 800150)
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