MML-1

Skin cancer cell lines

SKU: 300288 Category: Tags: ,

Description

categories-Skin cancer cell lines-cryovial-organism-Human-tissue-Skin-disease-Melanoma-synonyms-MML1-age-Unspecified-gender-Unspecified-ethnicity–morphology-Epithelial-like-cellType–growth Properties-Adherent-MML-1 (CLS catalog number 300288)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)

Brand

CYTION

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