Description
categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Thymus-disease-Leukemia-synonyms-L5178Y-R, L5178YR, L-5178-Y-R, LY-R, LYR-age–gender–ethnicity–morphology-Round cells-cellType-T lymphocyte-growth Properties-Suspension-L5178-R (CLS catalog number 400258)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate-subculturing-Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 x 10^4 cells/ml and maintain between 3 x 10^4 and 7 x 10^5 cells/ml.-freezeMedium-CM-ACF (CLS catalog number 800650)
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