CCRF-CEM

Description

categories- Leukemia cell lines-cryovial-organism-Human-tissue-Peripheral blood-disease-Leukemia-synonyms-CCRF/CEM, CCRFCEM, CCRF.CEM, CCRF CEM, CCRF, CEM, CEM-CCRF, CEM-CCRF (CAMR), CCRF/CEM/0, CEM/0, CEM-0, CCRF-CEM/S, GM03671, GM03671C-age-4 years-gender-Female-ethnicity-Caucasian-morphology-Polymorph cells, big nuclei, formation of microvilli-cellType-T lymphoblast-growth Properties-Suspension-CCRF-CEM (CLS catalog number 300147)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Subculture by diluting an appropriate volume of the cell suspension in a new flask containing fresh medium. Establish new cultures at 3 x 10^5 viable cells/ml. Upon thawing, culture in 1-2 T-25 cell culture flasks, incubate at 37 ?C/5% CO2. Renew the medium 24 hr later by centrifuging and resuspending the cells in the same amount of fresh medium unless the cell concentration exceeds 2 x 10^6 cells/ml.-freezeMedium-CM-ACF (CLS catalog number 800650)