Description
categories-B lymphoblastoid cell lines-cryovial-organism-Human-tissue-Spleen-disease-Hereditary spherocytosis-synonyms-WIL-2, Wil.2, WI-L2, Wi-L2-age-5 years-gender-Male-ethnicity-Caucasian-morphology–cellType-B lymphoblast-growth Properties-Suspension-WIL2 (CLS catalog number 302011)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Maintain culture between 3-9 x 10^5 cells/ml. A maximum density of 2 x10^6 cells/ml is possible. Incubate at 5% CO2, 37 ?C.-freezeMedium-CM-1 (CLS catalog number 800150)
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