Description

categories-Rat cell lines-cryovial-organism-Rat-tissue-Liver-disease-Hepatocellular carcinoma-synonyms-Novikoff-Hepatoma, NK-age–gender-Male-ethnicity–morphology–cellType–growth Properties-Suspension, some adherent cells-Novikoff Hepatoma (CLS catalog number 500373)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Resuspend the cell suspension and take a 100 ?l aliquot to count the cell number/ml. Dilute cell suspension to 1×10^5 cells/ml with fresh medium and transfer into new flasks.-freezeMedium-CM-ACF (CLS catalog number 800650)