AH-130

Description

categories-Rat cell lines-cryovial-organism-Rat-tissue-Liver-disease-Hepatocellular carcinoma-synonyms-Yoshida AH-130, Yoshida AH130, AH130, AH 130, AH-130 Yoshida, AH130-TC, AH130/P-age–gender–ethnicity–morphology-Round suspension cells, triangular adherent cells-cellType–growth Properties-Adherent/Suspension-AH-130 (CLS catalog number 500412)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-5% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. Using collagen-coated flasks increases the number of adherent cells.-freezeMedium-CM-ACF (CLS catalog number 800650)