Description
categories-Rat cell lines-cryovial-organism-Rat-tissue-Liver-disease-Hepatocellular carcinoma-synonyms-AH130FN-TC, AH130FN, AH-130F(N), AH-130FN, AH 130 FN-age–gender–ethnicity–morphology-Round cells in suspension, Epithelial-like when adherent-cellType–growth Properties-Suspension, few adherent-AH-130 FN (CLS catalog number 500451)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-5% FBS, 2 mM L-glutamine-subculturing-Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks.-freezeMedium-CM-1 (CLS catalog number 800150)
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