Description
categories-Rat cell lines-cryovial-organism-Rat-tissue-Liver-disease-Hepatocellular carcinoma-synonyms-A-S-30D, AS30D-age-16 months-gender-Female-ethnicity–morphology-Round cells, loosely adherent, floating-cellType–growth Properties-Suspension-AS-30D (CLS catalog number 500116)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose-subculturing-Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks.-freezeMedium-CM-1 (CLS catalog number 800150)
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