Description
categories- Avian cell lines-cryovial-organism-Chicken-tissue-Liver-disease-Hepatocellular carcinoma-synonyms-Leghorn Male Hepatoma cell line-age-16 months-gender-Male-ethnicity–morphology-Epithelial-like, Dendritic like.-cellType–growth Properties-Adherent/suspension. It takes a couple of days until cells grow in adherent colonies.-LMH (CLS catalog number 601411)-biosafety Level-1-cultureMedium-EMEM-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-LMH cells attach better to tissue culture vessels which have been precoated with Collagen. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)
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