Description
categories- Brain cancer cell lines-cryovial-organism-Human-tissue-Brain-disease-Glioblastoma-synonyms–age-78 years-gender-Female-ethnicity-Caucasian-morphology–cellType–growth Properties-Spheroid culture, partly adherent-NCH690 (CLS catalog number 300120)-biosafety Level-1-cultureMedium-GBM-MG-mediumSupplements–subculturing-Mechanical dissociation by pipetting (5-10 times) with an Eppendorf pipette and 1000 ?l tips after centrifugation of the cells (10min, 300g, 10?C). Detach adherent cells by adding Accutase for 5min at 37 ?C.-freezeMedium-CM-ACF (CLS catalog number 800650)
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