Description
categories-Gastric cancer cell lines-cryovial-organism-Human-tissue-Gastric-disease-Gastric adenocarcinoma-synonyms-HGC 27, HGC27-age-Unspecified-gender-Unspecified-ethnicity–morphology-Epithelial-like, polygonal or short spindle-shaped-cellType–growth Properties-Monolayer, adherent-HGC-27 (CLS catalog number 300436)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-5% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)
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