Description
categories-Soft tissue cancer cell lines-cryovial-organism-Human-tissue–disease-Fibrosarcoma-synonyms-Ht-1080, HT 1080, HT1080, HT 1080.T-age-35 years-gender-Male-ethnicity-Caucasian-morphology-Epithelial-like-cellType-Fibroblast-growth Properties-Adherent-HT-1080 (CLS catalog number 300216)-biosafety Level-1-cultureMedium-EMEM-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-ACF (CLS catalog number 800650)
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