KYSE-30

Description

categories-Head-Neck cancer cell lines-cryovial-organism-Human-tissue-Esophageal Squamous Epithelium-disease-Esophageal squamous cell carcinoma-synonyms-Kyse-30, KYSE 30, KYSE30, Kyse30, KYSE0030-age-64 years-gender-Male-ethnicity-Asian-morphology-Epithelial-Like, With Long Pseudopod-cellType–growth Properties-Adherent-KYSE-30 (CLS catalog number 305094)-biosafety Level-1-cultureMedium-Hams F12:RPMI 1640-mediumSupplements-10% FBS-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)