TF-1

Description

categories- Leukemia cell lines-cryovial-organism-Human-tissue-Bone marrow-disease-Erythroleukemia-synonyms-TF1, MFD-1-age-35 years-gender-Male-ethnicity-Japanese-morphology–cellType–growth Properties-Suspension-TF-1 (CLS catalog number 300434)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose, 10% KMG, for long-term culture: IL-3-subculturing-Start cultures at 2 x 10^5 cells/ml and maintain between 1 x 10^5 and 1 x 10^6 cells/ml. Subculture by transferring an aliquot of the cell suspension into a new cell culture flask already containing an appropriate amount of fresh cell culture medium.-freezeMedium-CM-1 (CLS catalog number 800150)