Description
categories- Leukemia cell lines-cryovial-organism-Human-tissue-Blood-disease-Chronic myeloid leukemia-synonyms–age-45 years-gender-Male-ethnicity-Caucasian-morphology–cellType-Undifferentiated blast cells-growth Properties-Suspension-BV-173 (CLS catalog number 300133)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Start new cultures at 1 x 10^5 viable cells/ml. Subculture when the cell concentration has reached 1 x 10^6 cells/ml. Prepare dilutions by transferring an appropriate volume of cell suspension into new flasks containing fresh cell culture medium. Optimal cell growth at 0.5 – 2 x 10^6 cells/ml.-freezeMedium-CM-ACF (CLS catalog number 800650)
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