Description
categories-Urinary bladder cancer cell lines-cryovial-organism-Human-tissue-Bladder-disease-Carcinoma-synonyms–age-68 years-gender-Male-ethnicity-Caucasian-morphology-Epithelial-like-cellType–growth Properties-Adherent-5637 (CLS catalog number 300105)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)
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