Description

categories-Leukemia cell lines-cryovial-organism-Human-tissue-Bone marrow-disease-Acute promyelocytic leukemia-synonyms-NB4, NB.4-age-23 years-gender-Female-ethnicity-Caucasian-morphology-Round cells-cellType-B lymphocyte-growth Properties-Suspension-NB-4 (CLS catalog number 300299)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Establish new cultures at 0.5 x 10^6 viable cells/ml and subculture at 1 x 10^6 cells/ml. Maximum cell density at 1-2 x 10^6 cells/ml. Prepare dilutions by transferring the appropriate amount of cell suspension into new flasks with fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)