Description

categories- Leukemia cell lines-cryovial-organism-Human-tissue-Blood-disease-Acute promyelocytic leukemia-synonyms-HL 60, HL.60, HL60-age-36 years-gender-Female-ethnicity-Caucasian-morphology-Round cells-cellType-Lymphoblast-growth Properties-Suspension-HL-60 (CLS catalog number 300209)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Cultures can be maintained by addition or replacement of medium. Start new cultures at 2 x 10^5 viable cells/ml and subculture at 1 x 10^6 cells/ml.-freezeMedium-CM-ACF (CLS catalog number 800650)