Description

categories-Canine cell lines-cryovial-organism-Dog-tissue-Normal Kidney-disease–synonyms-MDCK supertube, SuperTube-age-Adult-gender-Female-ethnicity–morphology-Epithelial-cellType–growth Properties-Adherent-Super Tube (CLS catalog number 305004)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-10% FBS, 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, 1.2 g/L sodium bicarbonate, 0.05 mM non-essential amino acids-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)