Description

categories-Rat cell lines-cryovial-organism-Rat-tissue–disease-Sarcoma-synonyms–age–gender–ethnicity–morphology–cellType-Fibroblast-growth Properties-Adherent/suspension-RY(ham) Yoshida Sarcoma (CLS catalog number 500415)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. It is recommended to culture these cells on collagen-coated flasks.-freezeMedium-CM-1 (CLS catalog number 800150)