Description

categories-Myeloma cell lines-cryovial-organism-Human-tissue-Peripheral blood-disease-Multiple Myeloma-synonyms-RPMI-8226, RPMI.8226, RPMI8226, RPMI no. 8226, RPMI no 8226, RPMI #8226, 8226, RPMI 8226/S, RPMI-8226S, RPMI8226/S, 8226/S, Roswell Park Memorial Institute 8226, GM02132, GM2132, GM 2132, GM02132C, Simpson-age-61 years-gender-Male-ethnicity–morphology-Round cells-cellType–growth Properties-Adherent/suspension-RPMI 8226 (CLS catalog number 300431)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Start new cultures at 5 x 10^5 viable cells/ml and subculture at 1-2 x 10^6 cells/ml. Prepare dilutions by transferring the appropriate amount of cell suspension into new flasks with fresh medium. Maximum cell density is at 1-2 x 10^6 cell/ml.-freezeMedium-CM-1 (CLS catalog number 800150)