Description

categories-Rat cell lines-cryovial-organism-Rat-tissue-Adrenal gland-disease-Pheochromocytoma-synonyms-PC 12, PC12-age-Unspecified-gender-Male-ethnicity-Japanese-morphology-Polygonal-cellType–growth Properties-Small clusters in suspension, poorly adherent, patches on collagen.-PC-12 (CLS catalog number 500311)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Suspension cells: Remove cells from substrate by pipetting with fresh medium. To obtain single cells, pass the suspension several times through a 22 gauge needle and dispense into new flasks. Growing on collagen: To remove adherent cells, use the following standard protocol. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.-freezeMedium-CM-ACF (CLS catalog number 800650)