Description

categories-NRK cells fluorescence tagged-cryovial-organism-Rat-tissue-Kidney-disease–synonyms-NRK IBB-DiHcRed1-age–gender–ethnicity–morphology-Fibroblast-like cells with fusiform shape-cellType–growth Properties-Monolayer, adherent-NRK-IBB-DiHcRed1 (CLS catalog number 500671)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS, 2 mM L-glutamine, 4.5 g/L glucose, Add G418 to culture medium at a final concentration of 0.5 mg/ml-subculturing-Remove medium and rinse with PBS. Add fresh 0.025% trypsin/0.02% EDTA solution at 37 ?C until cells detach (typically ~5 min). To remove trypsin, add fresh medium, transfer to a tube and centrifuge. Aspirate the supernatant, resuspend the cell pellet in culture medium and dispense into new flasks. Add G418 to culture medium at a final concentration of 0.5 mg/ml.-freezeMedium-CM-1 (CLS catalog number 800150)