Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Skin-disease–synonyms-MCA3D, MCa3D, MCA/3D, MCA 3D-age–gender-Female-ethnicity–morphology–cellType-Keratinocyte-growth Properties-Adherent-MCA-3D (CLS catalog number 400437)-biosafety Level-1-cultureMedium-Hams F12-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress(1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 15-20 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)