Description

categories-Pig cell lines-cryovial-organism-Sus Scrofa-tissue-Kidney-disease–synonyms-LLC-PK(1), LLC-PK-1, LLC PK-1, LLc-PK1, LLC PK1, LLCPK1, Lilly Laboratories Cell-Porcine Kidney 1-age-3-4 weeks-gender-Male-ethnicity–morphology-Epithelial-like-cellType–growth Properties-Adherent/suspension. It takes a couple of days until cells grow in adherent colonies.-LLC-PK1 (CLS catalog number 607264)-biosafety Level-1-cultureMedium-DMEM:Hams F12-mediumSupplements-5% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambiente temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-ACF (CLS catalog number 800650)