Description

categories- Human cell lines-cryovial-organism-Human-tissue-Kidney, cortex, proximal tubule-disease–synonyms-Hk-2, HK2, Human Kidney-2-age-Adult-gender-Male-ethnicity-European-morphology-Epithelial-cellType–growth Properties-Adherent-HK-2 (CLS catalog number 305021)-biosafety Level-1-cultureMedium-Keratinocyte Cell Line Medium (Catalog number 820506)-mediumSupplements-10% FBS, 0.05 mg/ml BPE 5 ng/ml EGF (must be supplemented seperately – not included in CLS Keratinocyte Cell Line Medium)-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)